|Product Aliases||Rabbit Anti-Streptavidin pAb; Anti Streptavidin|
|Description||Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ≈10-14 mol/L. The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
GenScript Streptavidin Antibody is developed in rabbit using purified Streptavidin and highly purified from rabbit antiserum by protein G resin.
|Immunogen||Purified recombinant streptavidin|
|Purification Method||Protein G chromatography|
|Formulation||5 mg/ml, lyophilized in PBS, pH7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 5.0 mg/ml.|
|Ig Subclass||Rabbit IgG|
|Specificity||GenScript Streptavidin Antibody is specific to streptavidin.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Note||Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.5-1 ug/ml
Dot blot: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Other applications: user-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
|Cat. No. A00621|