Catalog Products » Antibodies » Epitope Tag Antibodies » Rabbit Anti-Streptavidin
Cat. No.
Name
Size   
Price
Selected
A00621-5
5 mg
$69
A00621-10
10 mg
$120
Streptavidin Antibody, pAb, Rabbit
Full Name Streptavidin Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-Streptavidin pAb; Anti Streptavidin
Documents
Streptavidin Antibody, pAb, RabbitDocument-MSDS: 8004_20081105024457.PDF (PDF)
Streptavidin Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Description
Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ≈10-14 mol/L. The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

GenScript Streptavidin Antibody is developed in rabbit using purified Streptavidin and highly purified from rabbit antiserum by protein G resin.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Streptavidin Antibody is specific to streptavidin.
Formulation
5 mg/ml, lyophilized in PBS, pH7.4, containing 0.02% sodium azide
Immunogen
Purified recombinant streptavidin
Purification Method
Protein G chromatography
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 5.0 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.5-1 ug/ml
Dot blot: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Other applications: user-optimized
Conjugation
Unconjugated
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
  • 13871_20110907010130.PDF
  • 12S000521_A00621_COA.pdf
  • 13A000568_A00621_COA.pdf
  • Citation
    Snyder H.,et al. Nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system.Antimicrob Agents Chemother.2013Nov;

    Yonekita T.,et al. Development of a novel multiplex lateral flow assay using an antimicrobial peptide for the detection of Shiga toxin–producing Escherichia coli.J Microbiol Methods.2013Jun;93(3):251-6

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