Catalog Products » Antibodies » Epitope Tag Antibodies » T7-tag Antibody, pAb, Rabbit
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A00622-40
40 μg
$79 $47
A00622-100
100 μg
$198
T7-tag Antibody, pAb, Rabbitcustom antibody production
T7-tag Antibody, pAb, Rabbit
Full Name T7-tag Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-T7-tag pAb;
Documents
T7-tag Antibody, pAb, RabbitDocument-MSDS: 6574_20081013223441.PDF (PDF)
T7-tag Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
T7-tag Antibody, pAb, Rabbit zoomT7-tag Antibody, pAb, Rabbit
Description
Well-characterized antibodies with useful epitope tags have been widely used in the study of protein expression in various systems.
The T7 tag (MASMTGGQQMG) is a peptide with 11 amino acids encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein.
The T7 tag serves as a tag in many expression vectors including the pET system that is based on the efficient T7 RNA polymerase expression system.
GenScript Rabbit Anti-T7-tag Polyclonal Antibody is developed in rabbit using a synthetic T7-tag epitope peptide conjugated to KLH.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
This Antibody recognizes T7 tagged fusion proteins.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
T7-tag epitope peptide conjugated to KLH
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-3 µg/ml
Other applications: user-optimized
Conjugation
Unconjugated
Host Species
Rabbit
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
  • 13C000602_A00602 _COA.pdf
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