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V5-tag Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A00623-200	V5-tag Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A00623-40	V5-tag Antibody, pAb, Rabbit	40 μg	$50

Full Name

V5-tag Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-V5-tag pAb; Anti-V5-tag

Documents
Document-MSDS: 4154_20080228020120.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.

Concentration

0.5 mg/ml,lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Immunogen

V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Conjugation

Unconjugated

Host Species

Rabbit

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

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A00877-40	V5-tag Antibody [HRP], pAb, Rabbit	40 μg	$55
A00877-200	V5-tag Antibody [HRP], pAb, Rabbit	200 μg(5x40μg)	$248
A01724-40	THE^TM V5 Tag Antibody, mAb, Mouse	40 μg	$119
A01724-100	THE^TM V5 Tag Antibody, mAb, Mouse	100 μg	$219
A01724-200	THE^TM V5 Tag Antibody, mAb, Mouse	200 μg(5x40μg)	$395


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