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CBP-tag Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A00635-200	CBP-tag Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A00635-40	CBP-tag Antibody, pAb, Rabbit	40 μg	$50

Full Name

CBP-tag Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-CBP-tag pAb;

Documents
Document-MSDS: 4107_20080228003631.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
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Reference

Description

Well-characterized antibodies consisting of short sequences have been widely and effectively used in the study of protein expression in various systems.
CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2.
GenScript Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH.
CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting CBP fusion proteins.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript CBP-tag Antibody recognizes CBP tagged fusion proteins.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

A synthetic CBP tag peptide KRRWKKNFIAVSAANRFKKISSSGAL conjugated to KLH

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot:0.1-1.0 µg/ml
Western Blot Using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Mouse, rat, bovine and rabbit

Host Species

Rabbit

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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