Catalog Products » Antibodies » Epitope Tag Antibodies » CBP-tag Antibody, pAb, Rabbit
Cat. No.
Name
Size   
Price
Selected
A00635-40
40 μg
$79
A00635-200
200 μg
$275
A00635-100
100 μg
$198
CBP-tag Antibody, pAb, Rabbit
Full Name CBP-tag Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-CBP-tag pAb;
Documents
CBP-tag Antibody, pAb, RabbitDocument-MSDS: 4107_20080228003631.PDF (PDF)
CBP-tag Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
CBP-tag Antibody, pAb, Rabbit zoomCBP-tag Antibody, pAb, Rabbit
CBP-tag Antibody, pAb, Rabbit zoomCBP-tag Antibody, pAb, Rabbit
CBP-tag Antibody, pAb, Rabbit zoomCBP-tag Antibody, pAb, Rabbit
Description
CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2.
GenScript Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH. CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The antibody is suitable for detecting CBP fusion proteins.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript CBP-tag Antibody recognizes CBP tagged fusion proteins.
Formulation
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
A synthetic CBP tag peptide KRRWKKNFIAVSAANRFKKISSSGAL conjugated to KLH
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot:0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Immunoprecipitation: 1-5 µg/ml.
Other applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Mouse, rat, bovine and rabbit
Host Species
Rabbit
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
IF & ICC Protocol (pdf)
Document-COA
  • 13D000627_A00635_COA.pdf
  • 13G000657_A00635_COA.pdf
  • 13S000680_A00635_COA.pdf
  • Citation
    Park E.,et al. Structure of the SecY channel during initiation of protein translocation.Nature.2013Nov;

    van Bon BW.,et al. CEP89 is required for mitochondrial metabolism and neuronal function in man and fly.Hum Mol Genet.2013Apr;

    Wanschers BF.,et al. C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.Nucleic Acids Res.2012May;

    Thivierge C.,et al. Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi.Nat Struct Mol Biol.2011Dec;

    Cestari I.,et al. A Multiple Aminoacyl-tRNA Synthetase Complex That Enhances tRNA-Aminoacylation in African Trypanosomes.Mol Cell Biol.2013Dec;33(24):4872-88

    Szklarczyk R.,et al. A mutation in the FAM36A gene, the human ortholog of COX20, impairs cytochrome c oxidase assembly and is associated with ataxia and muscle hypotonia.Hum Mol Genet.2013Feb;22(4):656 - 67

    Jason Carnes.,et al. Mutational Analysis Of Trypanosoma Brucei Editosome Proteins Krepb4 And Krepb5 Reveals Domains Critical For Function.RNA.2012Otc;18(10):1897 - 1909

    Woods NT.,et al. Charting the landscape of tandem BRCT domain¯mediated protein interactions.Sci Signal.2012Sep;5(242):rs6

    Sejeong Shin.,et al. Glycogen Synthase Kinase (Gsk)-3 Promotes P70 Ribosomal Protein S6 Kinase (P70S6K) Activity And Cell Proliferation.Proc Natl Acad Sci U S A.2011Nov;108(47):E1204 - E1213

    Szklarczyk R.,et al. Iterative orthology prediction uncovers new mitochondrial proteins and identifies C12orf62 as the human ortholog of COX14, a protein involved in the assembly of cytochrome c oxidase.Genome Biol.2012Feb;13(2):12

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