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CBP-tag Antibody, pAb, Rabbit  |
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| Full Name |
CBP-tag Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-CBP-tag pAb; |
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Description |
Well-characterized antibodies consisting of short sequences have been widely and effectively used in the study of protein expression in various systems.
CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2.
GenScript Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH.
CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting CBP fusion proteins. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript CBP-tag Antibody recognizes CBP tagged fusion proteins. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
A synthetic CBP tag peptide KRRWKKNFIAVSAANRFKKISSSGAL conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot:0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Mouse, rat, bovine and rabbit |
Host Species |
Rabbit |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 4107_20080228003631.PDF (PDF)
