||Rabbit Anti-CBP-tag pAb;
||CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2.
GenScript Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH. CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The antibody is suitable for detecting CBP fusion proteins.
||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
||GenScript CBP-tag Antibody recognizes CBP tagged fusion proteins.
||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot:0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Immunoprecipitation: 1-5 µg/ml.
Other applications: user-optimized
Western Blot Protocol(pdf)
IF & ICC Protocol(pdf)
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