Protein C -tag Antibody (HPC4), pAb, Rabbit
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Protein C -tag Antibody (HPC4), pAb, Rabbit  |
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| Full Name |
Protein C -tag Antibody (HPC4), pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-Protein C (HPC4)-tag pAb; |
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Description |
Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein.Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.
Genscript Rabbit Anti-Protein C (HPC4)-tag antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
This Antibody recognizes Protein C tagged fusion proteins. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02%
sodium azide
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Immunogen |
Protein C epitope tag peptide CEDQVDPRLIDGK conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
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Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) (GenScript, L00204C) is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 4125_20080228012624.PDF (PDF)
