You are here: Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies » Rabbit Anti-AMPK1 (Ab-174) polyclonal antibody

AMPKα1/AMPKα2 Antibody (Ab-174/172), pAb, Rabbit

Full Name

Abbreviated name-1

AMPKα1/AMPKα2 Antibody (Ab-174/172), pAb, Rabbit;

Documents
Document-MSDS: 8046_20081106025716.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
zoom
zoom
zoom
Reference

Description

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-COA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-COA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Ig Subclass

Rabbit IgG

Storage

Store at -20°C/1 year.

Specificity

GenScript AMPKα1/AMPKα2 Antibody (Ab-174/172) detects endogenous levels of total AMPKα1/AMPKα2 protein.

Concentration

1 mg/ml in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol

Immunogen

Synthesized non-phosphopeptide derived from human AMPKα1/AMPKα2 around the phosphorylation site of threonine 174/172 (L-R-T^P-S-C).

Purification Method

GenScript Rabbit Anti-AMPKα1/AMPKα2 Antibody (Ab-174/172) Polyclonal Antibody was purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Predicted Band Size

63 kD

Observed Band Size

63 kD

Applications

IHC: 1:50-1:100
WB: 1:500-1:1,000
IF: 1:100-1:200

Conjugation

Unconjugated

Species Reactivity

Human, Mouse, Rat

Host Species

Rabbit

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.