||Rabbit Anti-Avi-tag pAb; Rabbit Anti-Avi-tag
||Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.
The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. GenScript Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.
||Avi tag peptide conjugated to KLH
||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
||This Antibody recognizes Avi tagged fusion proteins.
||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Western Blot Protocol(pdf)
Lam MT.,et al. Rev-Erbs repress macrophage gene expression by inhibiting enhancer-directed transcription.Nature.2013Jun;
Janes PW.,et al. Eph Receptor Function Is Modulated By Heterooligomerization Of A And B Type Eph Receptors.J Cell Biol.2011Dec;
JD Stender.,et al. Control Of Proinflammatory Gene Programs By Regulated Trimethylation And Demethylation Of Histone H4K20.Mol Cell.2012Dec;48(1):28-38
Sung K.,et al. A novel method for producing mono-biotinylated, biologically active neurotrophic factors: An essential reagent for single molecule study of axonal transport.J Neurosci Methods.2011Sep;200(2):121-8
J Neef, et al. Efficient production of secreted staphylococcal antigens in a non-lysing and proteolytically reduced Lactococcus lactis strain. Appl Microbiol Biotechnol. 2014Sep.;