Catalog Products » Antibodies » Epitope Tag Antibodies » Universal-tag Antibody, pAb, Rabbit
Cat. No.
Name
Size   
Price
Selected
A00680-40
40 μg
$79 $47
A00680-100
100 μg
$198
Universal-tag Antibody, pAb, Rabbit
Full Name Universal-tag Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti Universal-tag pAb; Anti Universal-tag pAb
Documents
Universal-tag Antibody, pAb, RabbitDocument-MSDS: 4231_20080228215511.PDF (PDF)
Universal-tag Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
Universal-tag Antibody, pAb, Rabbit zoomUniversal-tag Antibody, pAb, Rabbit
Universal-tag Antibody, pAb, Rabbit zoomUniversal-tag Antibody, pAb, Rabbit
Description
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. Universal-tag is an epitope tag composed of a six-residue peptide, HTTPHH. GenScript Rabbit Anti-Universal-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
This Antibody recognizes Universal tagged fusion proteins.
Formulation
1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide
Immunogen
Universal epitope tag peptide HTTPHH conjugated to KLH
Purification Method
Immunoaffinity-purified
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit:
For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized
Conjugation
Unconjugated
Host Species
Rabbit
Note
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.