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MAT-tag Antibody, pAb, Rabbit  |
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| Full Name |
MAT-tag Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-MAT-tag pAb; Anti-MAT-tag pAb |
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Description |
Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The MAT tag (Metal Affinity Tag) is a peptide tag that binds to transition metals such as nickel and cobalt. This tag allows the purification of MAT fusion proteins using immobilized metal affinity chromatography. GenScript Rabbit Anti-MAT-tag polyclonal antibody is developed in rabbit using a synthetic MAT tag peptide conjugated to KLH.
Rabbit Anti-MAT-tag polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
This Antibody recognizes MAT tagged fusion proteins. |
Concentration |
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide |
Immunogen |
Synthetic peptide conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Applications |
Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2.0 µg/ml
Western blot using the GenScript ONE-HOUR WesternTM Kit:
For quick results, we recommend the GenScript ONE-HOUR WesternTM Kit (L00204C). 15 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8070_20081107011546.PDF (PDF)
