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β-Amyloid Antibody (32-40), pAb, Rabbit

Full Name

Abbreviated name-1

Rabbit Anti-β-Amyloid (32-40) pAb;

Documents
Document-MSDS: 4216_20080228040053.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

A number of mutations, identified in the gene encoding the β-amyloid precursor protein (βAPP), have been linked to early-onset Familial Alzheimers Disease. βAPP is cleaved sequentially by the proteolytic enzymes β-secretase and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42(43) and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides can bind to scavenger receptors and the receptor for advanced glycation endproducts. Aβ peptides are degraded either via a reuptake mechanism followed by endosomal degradation or by an extracellular insulin-degrading enzyme. Extracellular accumulation of Aβ leads to formation of aggregates, fibrils, and eventually amyloid deposits called neuritic plaques, a hallmark of Alzheimerâs disease. β-Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimers disease.

GenScript Rabbit Anti-β-Amyloid (32-40) Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide corresponding to amino acids 32-40 of human β-Amyloid conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.

Ig Subclass

Rabbit IgG

Storage

GenScript Rabbit Anti-β-Amyloid (32-40) Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-β-Amyloid (32-40) is specific to human β-amyloid 1-40. Do not expect it to react with human β-amyloid 1-42

Concentration

1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

A synthetic peptide corresponding to amino acids 32-40 of β-Amyloid conjugated to KLH

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1.0 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/mlImmunofluorescence: 5-20 µg/ml
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.