Amyloid Precursor Protein Antibody (44-63), pAb, Rabbit
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Amyloid Precursor Protein Antibody (44-63), pAb, Rabbit  |
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| Full Name |
Amyloid Precursor Protein Antibody (44-63), pAb, Rabbit
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Abbreviated name-1 |
Amyloid Precursor Protein antibody; APP antibody |
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Description |
Amyloid precursor protein (APP) is a type I integral membrane protein with a large N-terminal extracellular domain, a single transmembrane domain, and a short cytoplasmic tail. APP is found as three main isoforms, APP695, APP751, and APP770. APP can be sequentially cleaved by beta-secretase and gamma-secretase to release beta-amyloid, which is the main component of senile plaque in patient brains inflicted with Alzheimer disease.
GenScript Rabbit Anti-APP (44-63) Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide, HMNVQNGKWDSDPSGTKTCI, corresponding to N-terminal amino acids 44-63 of human APP conjugated to KLH. GenScript Rabbit Anti-APP (44-63) Polyclonal Antibody is a valuable tool for scientists and neuropathologists who study the role of APP in Alzheimer disease.
GenScript Rabbit Anti-APP (44-63) Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-APP (44-63) can detect human recombinant APP, endogenous APP from cell lysates and tissue slides, and mouse APP in brain tissue lysate. |
Concentration |
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide |
Immunogen |
A synthetic peptide HMNVQNGKWDSDPSGTKTCI conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunofluorescence: 5-20 µg/ml
Western Blot Using the GenScript ONE-HOUR WesternTM Kit: For quick results, we recommend the GenScript ONE-HOUR WesternTM Complete Kit (Rabbit) (L00204C). 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse
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Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8074_20081107023705.PDF (PDF)
