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Amyloid Precursor Protein Antibody (747-761), pAb, Rabbit

Cat. No.	Name	Size	Price
A00696-200	Amyloid Precursor Protein Antibody (747-761), pAb, Rabbit	200 μg(5x40μg)	$225
A00696-40	Amyloid Precursor Protein Antibody (747-761), pAb, Rabbit	40 μg	$50

Full Name

Amyloid Precursor Protein Antibody (747-761), pAb, Rabbit

Abbreviated name-1

Rabbit Anti-Amyloid Precursor Protein pAb;

Documents
Document-MSDS: 8082_20081107034106.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
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Reference

Description

Amyloid precursor protein (APP) is a type I integral membrane protein with a large N-terminal extracellular domain, a single transmembrane domain, and a short cytoplasmic tail. APP is found as three main isoforms, APP695, APP751 and APP770. APP can be sequentially cleaved by beta-secretase and gamma-secretase to release beta-amyloid, which is the main component of senile plaque in patient brains inflicted with Alzheimers disease.
GenScript Rabbit Anti-APP (747-761) Polyclonal Antibody is developed in rabbit using a synthetic peptide, RHLSKMQQNGYENPT, corresponding to the C term amino acids 747-761 of human APP. GenScript Rabbit anti APP (747-761) Polyclonal Antibody should be a valuable tool for scientists and neuropathologists who study the role of APP in Alzheimerâs disease.
Rabbit Anti-APP (747-761) Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-APP (747-761) Polyclonal Antibody is specific to human APP.

Concentration

0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide

Immunogen

Synthetic peptide, RHLSKMQQNGYENPT, corresponding to the C term amino acids 747-761 of human APP, conjugated to KLH.

Purification Method

Immunoaffinity chromatography

Applications

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human, mouse

Host Species

Rabbit

Antigen Species

Human

Note

Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.