APP FM Antibody (339-348), pAb, Rabbit
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APP FM Antibody (339-348), pAb, Rabbit  |
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| Full Name |
APP FM Antibody (339-348), pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-APP FM (339-348) pAb; |
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Description |
Amyloid precursor protein (APP) is a type I integral membrane protein with a large N-terminal extracellular domain which can be sequentially cleaved by beta-secretase and gamma-secretase to release beta-amyloid. The extracellular deposition and accumulation of the released beta-amyloid fragments form the main components of amyloid plaques in Alzheimerâs disease. APP is also found as a truncated frameshift mutant, which results from a GA dinucleotide deletion from the normal APP gene. This abnormal protein with a molecular weight of about 38 kD has been found to accumulate in the brains of some patients with Alzheimers disease. The role of the Amyloid Precursor Protein frameshift mutant (APP FM) in Alzheimers disease is currently unknown. These abnormal proteins may accumulate and lead to cellular disturbances and neuronal degeneration.
GenScript Rabbit Anti-APP FM Polyclonal Antibody is developed in rabbit using a synthetic peptide, RGRTSSKELA, corresponding to amino acids 339-348 of the C-terminus of human APP frameshift mutant.
GenScript Rabbit Anti-APP FM Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-APP FM Polyclonal Antibody can detect endogenous level of APP FM. |
Concentration |
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide |
Immunogen |
Synthetic peptide corresponding to amino acids 339-348 (RGRTSSKELA) of human APP frameshift mutant conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/ml
Western blot: 0.1-1.0 μg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8090_20081109210636.PDF (PDF)