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IFN-γ Antibody, mAb, Mouse

Full Name

Abbreviated name-1

Mouse Anti-IFN-γ mAb;

Documents
Document-MSDS: 8108_20081110043102.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
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Reference
Tsubota, K. et al. Invest. Ophthal. Vis. Sci. 40: 28-34, 1999. Takayanagi, H. et al. Nature 2000. 408: 600-605.

Description

Interferon-γ (IFN-γ) is a pro-inflammatory cytokine that is central in host resistance to infection. It is mainly produced by natural killer cells and CD4+ and CD8+ T cells. Its receptors are found on nearly all cells, where it activates diverse responses that enable potential host cells to prevent invasive infection by bacteria, parasites, and viruses. Takayanagi et al. (2000) demonstrated that IFN-γ strongly suppresses osteoclastogenesis by interfering with the RANKL (602642)-RANK (603499) signaling pathway. Tsubota et al. (1999) reported that this upregulation in Sjogren syndrome patients may be controlled by interferon-gamma through the activation of transcription factor NFKB.

Ig Subclass

IgG1

Storage

GenScript Mouse Anti-IFN-γ Monoclonal Antibody remains stable for one to two weeks if stored at 2°C to 8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. Use within one year of the date of receipt.

Specificity

Truncated IFN-γ recombinant protein (WB), human IFN-γ recombinant protein (ELISA)

Concentration

1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide

Immunogen

Ni-NTA purified recombinant human IFN-γ expressed in E. coli strain BL21 (DE3)

Purification Method

GenScript Mouse Anti-IFN-γ Monoclonal Antibody is purified by protein A affinity chromatography.

Applications

Anti-IFN-γ recognizes recombinant IFN-γ in Western blot and ELISA.
Western blot: 1:500-1:1,000
ELISA: Determining optimal working dilutions by titration test

Host Species

Mouse

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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