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CD14 Antibody, mAb, Mouse

Full Name

Abbreviated name-1

Mouse Anti-Human CD14 mAb;

Documents
Document-MSDS: 8127_20081111005253.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
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Reference
Richert MM, Schwertfeger KL, Ryder JW, Anderson SM: An atlas of mouse mammary gland development. J Mammary Gland Biol Neoplasia 2000, 5 : 227-241. Masso-Welch PA, Darcy KM, Stangle-Castor NC, Ip MM: A developmental atlas of rat mammary gland histology. J Mammary Gland Biol Neoplasia 2000, 5 : 165-185.

Description

CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55,000 Da. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages, and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serve two physiological roles. These proteins act as opsonin and opsonic receptors, respectively, promoting the phagocytic uptake of bacteria and LPS-coated particles by macrophages.

Ig Subclass

IgG1

Storage

GenScript Mouse Anti-Human CD14 Monoclonal Antibody remains stable for one to two weeks if stored at 2°C to 8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. Use within one year of the date of receipt.

Specificity

Human lymph node tissue(IHC), human CD14 recombinant protein (ELISA)

Concentration

1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide

Immunogen

Ni-NTA purified recombinant human CD14 expressed in E. coli strain BL21 (DE3)

Purification Method

GenScript Mouse Anti-Human CD14 Monoclonal Antibody is purified by protein G affinity chromatography and resuspended in 10 mM PBS (pH 7.6) and 0.02% sodium azide. No reconstitution is necessary.

Applications

IHC: Dilution 1:200-1:400
Determining optimal working dilutions by titration test

Host Species

Mouse

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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