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EphB6 Antibody, mAb, Mouse  |
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| Full Name |
EphB6 Antibody, mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-EphB6 mAb; |
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Description |
EPH receptor B6 (EphB6) is an 1006-amino acid protein of about 110,000 Da. It belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Eph receptors and ephrin ligands are membrane-bound cell-cell communication molecules with well-defined functions in development. EphB6 is expressed in a variety of both embryonic and adult tissues. EphB6 is a unique member in the Eph family of receptor tyrosine kinases in that its kinase domain contains several alterations in conserved amino acids and is catalytically inactive. EphB6 can both positively and negatively regulate cell adhesion and migration and tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. |
Ig Subclass |
IgG1 |
Storage |
GenScript Mouse Anti-EphB6 Monoclonal Antibody remains stable for one to two weeks if stored at 2°C to 8°C. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. Use within one year of the date of receipt. |
Specificity |
Jurkat/NIH 3T3 cell extracts (WB), human EphB6 recombinant protein (ELISA), human bladder carcinoma / rectum carcinoma (ELISA) |
Concentration |
1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide |
Immunogen |
Ni-NTA purified truncated recombinant EphB6 expressed in E. coli strain BL21 (DE3) |
Applications |
Western blot: 1:500-1:2,000
IHC(P): 1:500-1:2,000
IHC(F): 1:500-1:2,000
ELISA: Proposed dilution 1:10,000
Determining optimal working dilutions by titration
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Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8139_20081111024300.PDF (PDF)
