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Cat. No.
Name
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A00774
40 μg
$55
BRAF Antibody, mAb, Mousecustom antibody production
BRAF Antibody, mAb, Mouse
Full Name BRAF Antibody, mAb, Mouse
Abbreviated name-1
Mouse Anti-BRAF mAb;
Documents
BRAF Antibody, mAb, MouseDocument-MSDS: 8178_20081112034348.PDF (PDF)
BRAF Antibody, mAb, MouseDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
BRAF Antibody, mAb, Mouse zoomBRAF Antibody, mAb, Mouse
BRAF Antibody, mAb, Mouse zoomBRAF Antibody, mAb, Mouse
Citations
Vida Khatamianfar.,et al. Trim59, A Novel Multiple Cancer Biomarker For Immunohistochemical Detection Of Tumorigenesis.BMJ Open.2012Otc;2(5):e001410

Description
BRAF (V-raf murine sarcoma viral oncogene homolog B1) is the main effector recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites, Ser364, Ser428, and Thr439. B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK). It has a long amino-terminal region, which is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf can propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testes. Defects in BRAF are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas, and colorectal cancers.
Ig Subclass
IgG1
Storage
This product remains stable for one to two weeks if stored at 2°C to 8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. Use within one year of the delivery date.
Specificity
Truncated recombinant BRAF/K562 cell lysate (WB), BRAF recombinant protein (ELISA), Human bladder/lung carcinoma tissue (IHC)
Concentration
Ascitic fluid containing 0.03% sodium azide
Immunogen
Ni-NTA purified truncated recombinant BRAF expressed in E. coli strain BL21 (DE3).
Clone ID
A00774.01
Applications
Western blot: 1: 500-1:1,000
IHC(P): 1:500-1:1,000
ELISA: Proposed dilution 1:10,000
Determine optimal working dilutions by titration test.
Host Species
Mouse
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.