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AURKB Antibody, mAb, Mouse

Full Name

Abbreviated name-1

Mouse Anti-AURKB mAb;

Documents
Document-MSDS: 8191_20081112215725.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
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Reference
Song J et al. Immunity 22:621-31 (2005). Kapoor P et al. Mol Cell Biol 25:4934-45 (2005).

Description

AURKB (aurora kinase B, AIK2 or aurora-B), a 344-amino acid protein of about 39,000 Da, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is a mitotic protein kinase, which phosphorylates histone H3 and regulates chromosomal segregation during mitosis and meiosis. It may regulate several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis. It is also a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. ARK-2 transcripts are present at high levels in human thymus and fetal liver. ARK-2 protein levels are maximal during both S and G2/M phases.

Ig Subclass

IgG1

Storage

GenScript Mouse Anti-AURKB remains stable for one to two weeks if stored at 2°C to 8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. Use within one year of the date of receipt.

Specificity

Truncated AURKB recombinant protein / SK-N-SH cell lysate (WB), AURKB recombinant protein (ELISA)

Concentration

1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide

Immunogen

Ni-NTA purified truncated recombinant AURKB expressed in E. coli strain BL21 (DE3)

Applications

Western blot: 1:500-1:2,000
ELISA: Proposed dilution 1:10,000
Other applications: Determining optimal working dilutions by titration.

Host Species

Mouse

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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