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CK1 Antibody, mAb, Mouse  |
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| Full Name |
CK1 Antibody, mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-CK1 mAb; |
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Description |
CK1 (also designated Cytokeratin 1 or KRT1), with 644-amino acid protein (about 70,000 Da), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. |
Ig Subclass |
IgG1 |
Storage |
This product remains stable for one to two weeks if stored at 2°C to 8°C. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. Use before is one year from the date of receipt. |
Specificity |
Truncated CK1 recombinant protein (WB), human CK1 recombinant protein (ELISA), human liver tissue / colon carcinoma / lung carcinoma / esophagus tissue (IHC) |
Concentration |
1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide |
Immunogen |
Ni-NTA purified truncated recombinant CK1 expressed in E. coli strain BL21 (DE3) |
Applications |
Western blot: 1:500-1:2,000
IHC(P): 1:500-1:2,000
IHC(F): 1:500-1:2,000
ELISA: proposed dilution 1:10,000
Determining optimal working dilutions by titration test |
Host Species |
Mouse |
Antigen Species |
Human |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8229_20081114021606.PDF (PDF)
