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Vimentin Antibody, mAb, Mouse

Full Name

Abbreviated name-1

Mouse Anti-Vimentin mAb;

Documents
Document-MSDS: 8241_20081114040356.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Ig Subclass

IgG1

Storage

For long-term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. Use before is one year from the date of receipt.

Specificity

Vimentin recombinant protein (WB), human Vimentin recombinant protein (ELISA), human lung carcinoma tissue (IHC)

Concentration

1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide

Immunogen

Ni-NTA purified truncated recombinant Vimentin expressed in E. coli strain BL21 (DE3)

Purification Method

Mouse Anti-Vimentin is purified by protein A affinity chromatography

Applications

ELISA: Propose dilution 1:10,000
Determining optimal working dilutions by titration test

Host Species

Mouse

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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