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HPRT Antibody, mAb, Mouse  |
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| Full Name |
HPRT Antibody, mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-HPRT mAb; |
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Description |
The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA. The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C.2.4.2.8., HPRT) plays a crucial role in uric acid synthesis and purine metabolism. This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy. This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA. |
Ig Subclass |
IgG2b |
Storage |
This product remains stable for one to two weeks if stored at 2°C to 8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. Use within one year of the delivery date. |
Specificity |
Truncated HPRT recombinant protein (WB), HPRT recombinant protein (ELISA) |
Concentration |
Ascitic fluid containing 0.03% sodium azide |
Immunogen |
Ni-NTA purified truncated recombinant HPRT expressed in E. coli strain BL21 (DE3) |
Applications |
Western blot: 1:500-1:2,000
ELISA: 1:10,000
Determine optimal working dilutions by titration test. |
Conjugation |
Unconjugated |
Host Species |
Mouse |
Antigen Species |
Human |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8277_20081117042337.PDF (PDF)