Cat. No.
Name
Size   
Price
Selected
A00842
40 μg
$55
HPRT Antibody, mAb, Mouse
Full Name HPRT Antibody, mAb, Mouse
Abbreviated name-1
Mouse Anti-HPRT mAb;
Documents
HPRT Antibody, mAb, MouseDocument-MSDS: 8277_20081117042337.PDF (PDF)
HPRT Antibody, mAb, MouseDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
HPRT Antibody, mAb, Mouse zoomHPRT Antibody, mAb, Mouse
Description
The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA. The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C.2.4.2.8., HPRT) plays a crucial role in uric acid synthesis and purine metabolism. This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy. This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA.
Ig Subclass
IgG2b
Storage
This product remains stable for one to two weeks if stored at 2°C to 8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. Use within one year of the delivery date.
Specificity
Truncated HPRT recombinant protein (WB), HPRT recombinant protein (ELISA)
Formulation
Ascitic fluid containing 0.03% sodium azide
Immunogen
Ni-NTA purified truncated recombinant HPRT expressed in E. coli strain BL21 (DE3)
Clone ID
A00842.01
Applications
Western blot: 1:500-1:2,000
ELISA: 1:10,000
Determine optimal working dilutions by titration test.
Conjugation
Unconjugated
Host Species
Mouse
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
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2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.