You are here: Products » Antibodies » THE^TM Elite Antibodies » cMyc Tag Antibody » Mouse Anti-c-Myc [Biotin] Monoclonal Antibody

THE^TM c-Myc Antibody [Biotin], mAb, Mouse

Full Name

Abbreviated name-1

Mouse Anti-c-Myc [Biotin] mAb

Documents
MSDS: 20081117215820 (PDF)
MSDS: 20080118034804 (PDF)
Figures
zoom
Reference

Description

GenScript THE^TM c-Myc Antibody [Biotin], mAb, Mouse is the biotin conjugated THE^TM Anti-c-Myc Monoclonal Antibody (Mouse) (A00704) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

Ig Subclass

IgG2a, k

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript THE^TM c-Myc Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide

Clone

2G8D5

Immunogen

A synthetic peptide EQKLISEEDL coupled to KLH

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized antibody (40 µg) with 80 µl of deionized water (or equivalent) to the final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized

Fusion Partner

Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells

Conjugation

Biotin

Host Species

Mouse

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.

THETM c-Myc Antibody [Biotin], mAb, Mouse

THE^TM c-Myc Antibody [Biotin], mAb, Mouse