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THETM GST Antibody, mAb, Mouse


Select
Cat. No.
Product Name
Size
Price
Quantity
A00865-500 THETM GST Antibody, mAb, Mouse
500 μg
$750.00
A00865-40 THETM GST Antibody, mAb, Mouse
40 μg
$69.00
A00865-200 THETM GST Antibody, mAb, Mouse
200 μg
$311.00
A00865-100 THETM GST Antibody, mAb, Mouse
100 μg
$175.00

 


Abbreviated name-1 Mouse Anti-GST monoclonal antibody;
Description Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
Immunogen Recombinant GST protein (26 kD)
Host Species Mouse
Conjugation Unconjugated
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Method Protein A affinity column

Formulation 1. 40 µg, 100 µg, 200 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
2. 500 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, without sodium azide or carrier protein.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Clone ID 2F10B9
Ig Subclass IgG1, k
Specificity THETM Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2 ml of PBST).
Immunoprecipitation (IP): 1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

 THE<sup>TM</sup> GST Antibody, MAb, Mouse
 THE<sup>TM</sup> GST Antibody, MAb, Mouse

Western blot analysis of GST-tagged fusion proteins using THETM GST Antibody, mAb, Mouse (GenScript, A00865, 1 µg/ml)
Lane 1: N-terminal GST tagged fusion protein
Lane 2: Internal GST tagged fusion protein
Lane 3: C-terminal GST tagged fusion protein
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

 THE<sup>TM</sup> GST Antibody, MAb, Mouse
 THE<sup>TM</sup> GST Antibody, MAb, Mouse

Western blot analysis of GST-tagged fusion protein using THETM GST Antibody, mAb, Mouse (GenScript, A00865, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (GenScript, L00205)
Predicted Size: 26 kD
Observed Size: 26 kD

 THE<sup>TM</sup> GST Antibody, MAb, Mouse
 THE<sup>TM</sup> GST Antibody, MAb, Mouse

Western blot analysis of GST-tagged fusion protein using THETM GST Antibody, mAb, Mouse (GenScript, A00865 A: 1 µg/ml, B: 0.1 µg/ml)
Lane 1-3: GST tagged fusion protein
Lane 4: E. coli cell lysate
The signal was developed with One-Step WesternTM Basic Kit (GenScript, L00205).
Predicted Size: 26 kD
Observed Size: 26 kD

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse

 THE<sup>TM</sup> GST Antibody, MAb, Mouse


Cat. No.
Product Name
Size
Price
Quantity
A00130-100 GST-tag Antibody [HRP], pAb, Rabbit
100 μg
$199.00
A00202-100 GST-tag Antibody [Biotin], pAb, Rabbit
100 μg
$199.00
A00202-40 GST-tag Antibody [Biotin], pAb, Rabbit
40 μg
$99.00
A00865-100 THETM GST Antibody, mAb, Mouse
100 μg
$175.00
A00865-200 THETM GST Antibody, mAb, Mouse
200 μg
$311.00
A00865-500 THETM GST Antibody, mAb, Mouse
500 μg
$750.00
A00867-40 THETM GST Antibody [Biotin], mAb, Mouse
40 μg
$74.00
A01380-40 GST-tag Antibody [HRP], pAb, Goat
40 μg
$99.00
L00207 GST Fusion Protein Purification Kit
1 kit
$139.00
L00411 GST tag ELISA Detection Kit
1 kit (1x96 well)
$298.00
A00866-100 THETM GST Antibody [HRP], mAb, Mouse
100 µg
$180.00
A00097-100 GST-tag Antibody, pAb, Rabbit
100 μg
$199.00
A01379-100 GST-tag Antibody, pAb, Goat
100 μg
$199.00
 
Swaney DL., et al. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. 2013 Jul;10(7):676-82.

You can visit "Citations Database" for more citations. Muzammel Haque and Konstantin G. Kousoulas. The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Binds to HIF-1α and Causes Its Degradation via the Proteasome Pathway. J Virol. 2013 Feb;87(4):2164 - 2173.

You can visit "Citations Database" for more citations. Ghai R.,et al. Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.Proc Natl Acad Sci U S A.2013Feb;110(8):E643 - 52

You can visit "Citations Database" for more citations. Tsai KL.,et al. A conserved Mediator–CDK8 kinase module association regulates Mediator–RNA polymerase II interaction.Nat Struct Mol Biol.2013May;20(5):611-9

You can visit "Citations Database" for more citations. Z Lin, et al. Classical swine fever virus (CSFV) and p7 protein induce secretion of IL-1β in Macrophages. J Gen Virol. 2014Aug.;

You can visit "Citations Database" for more citations.







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