Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
1. 40 µg, 100 µg, 200 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. 2. 500 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, without sodium azide or carrier protein.
Recombinant GST protein (26 kD)
Protein A affinity column
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1 µg/ml Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2 ml of PBST).
Immunoprecipitation (IP): 1 µg/ml Other applications: user-optimized
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Swaney DL., et al. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. 2013 Jul;10(7):676-82.
Muzammel Haque and Konstantin G. Kousoulas. The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Binds to HIF-1α and Causes Its Degradation via the Proteasome Pathway. J Virol. 2013 Feb;87(4):2164 - 2173.
Ghai R.,et al. Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.Proc Natl Acad Sci U S A.2013Feb;110(8):E643 - 52
Tsai KL.,et al. A conserved Mediator–CDK8 kinase module association regulates Mediator–RNA polymerase II interaction.Nat Struct Mol Biol.2013May;20(5):611-9
Z Lin, et al. Classical swine fever virus (CSFV) and p7 protein induce secretion of IL-1β in Macrophages. J Gen Virol. 2014Aug.;