Catalog Products » Antibodies » THE™ Elite Antibodies » THETM GST Antibody, mAb, Mouse
Email to GenScript

Cat. No.
Product Name
Size
Price
Quantity
A00865-500 THETM GST Antibody, mAb, Mouse
500 μg
$750.00
A00865-40 THETM GST Antibody, mAb, Mouse
40 μg
$69.00
A00865-200 THETM GST Antibody, mAb, Mouse
200 μg
$311.00
A00865-100 THETM GST Antibody, mAb, Mouse
100 μg
$175.00
 THE<sup>TM</sup> GST Antibody, mAb, Mouse
Full Name
THETM GST Antibody, mAb, Mouse  THE<sup>TM</sup> GST Antibody, mAb, Mouse
Abbreviated name-1
Mouse Anti-GST monoclonal antibody;
Documents
 THE<sup>TM</sup> GST Antibody, mAb, MouseDocument-MSDS: A00865_MSDS.pdf (pdf)
 THE<sup>TM</sup> GST Antibody, mAb, MouseDocument-MSDS: Sodium_Azide_MSDS.pdf (pdf)
Figures
 THE<sup>TM</sup> GST Antibody, MAb, Mouse zoom THE<sup>TM</sup> GST Antibody, MAb, Mouse
Figures
 THE<sup>TM</sup> GST Antibody, MAb, Mouse zoom THE<sup>TM</sup> GST Antibody, MAb, Mouse
Figures
 THE<sup>TM</sup> GST Antibody, MAb, Mouse zoom THE<sup>TM</sup> GST Antibody, MAb, Mouse
Description
Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
Ig Subclass
IgG1, k
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
THETM Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Formulation
1. 40 µg, 100 µg, 200 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
2. 500 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, without sodium azide or carrier protein.
Immunogen
Recombinant GST protein (26 kD)
Clone ID
2F10B9
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2 ml of PBST).
Immunoprecipitation (IP): 1 µg/ml
Other applications: user-optimized
Fusion Partner
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Conjugation
Unconjugated
Host Species
Mouse
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)
Document-COA
  • 10K000193_A00865_COA
  • 11I000353_A00865_COA
  • 12C000469_A00865_COA
  • 13S000676_A00865_COA.pdf
  • 14G000818_A00865_COA.pdf
  • Citation
    Swaney DL., et al. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. 2013 Jul;10(7):676-82.

    Muzammel Haque and Konstantin G. Kousoulas. The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Binds to HIF-1α and Causes Its Degradation via the Proteasome Pathway. J Virol. 2013 Feb;87(4):2164 - 2173.

    Ghai R.,et al. Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.Proc Natl Acad Sci U S A.2013Feb;110(8):E643 - 52

    Tsai KL.,et al. A conserved Mediator–CDK8 kinase module association regulates Mediator–RNA polymerase II interaction.Nat Struct Mol Biol.2013May;20(5):611-9

    Z Lin, et al. Classical swine fever virus (CSFV) and p7 protein induce secretion of IL-1β in Macrophages. J Gen Virol. 2014Aug.;

    Related Products
    Cat. No.
    Product Name
    Size
    Price
    Quantity
    A00130-40 GST-tag Antibody [HRP], pAb, Rabbit
    40 μg
    $99.00
    A00202-100 GST-tag Antibody [Biotin], pAb, Rabbit
    100 μg
    $199.00
    A00202-40 GST-tag Antibody [Biotin], pAb, Rabbit
    40 μg
    $99.00
    A00865-40 THETM GST Antibody, mAb, Mouse
    40 μg
    $69.00
    A00865-200 THETM GST Antibody, mAb, Mouse
    200 μg
    $311.00
    A00865-500 THETM GST Antibody, mAb, Mouse
    500 μg
    $750.00
    A00866-40 THETM GST Antibody [HRP], mAb, Mouse
    40 μg
    $74.00
    A00867-200 THETM GST Antibody [Biotin], mAb, Mouse
    200 μg
    $333.00
    A01380-40 GST-tag Antibody [HRP], pAb, Goat
    40 μg
    $99.00
    L00207 GST Fusion Protein Purification Kit
    1 kit
    $139.00
    A00097-100 GST-tag Antibody, pAb, Rabbit
    100 μg
    $199.00
    A01380-100 GST-tag Antibody [HRP], pAb, Goat
    100 μg
    $199.00
    1
    Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
    2
    Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
    3
    Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.