THETM GST Antibody [HRP], mAb, Mouse
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THE TM GST Antibody [HRP], mAb, Mouse  |
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| Full Name |
THETM GST Antibody [HRP], mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-GST [HRP] mAb; |
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Description |
GenScript THETM GST Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of GST in fusion proteins. |
Ig Subclass |
IgG1
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Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
THETM GST Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins. |
Concentration |
0.5mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
Immunogen |
Recombinant GST protein (26 kD) |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05–0.2 µg/ml Western blot: 0.1-1 µg/ml Other applications: user-optimized
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Fusion Partner |
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells |
Conjugation |
Horseradish peroxidase (HRP) |
Host Species |
Mouse |
Technical Manual |
TM0589.pdf (PDF) TM0590.pdf (PDF) TM0591.pdf (PDF) TM0592.pdf (PDF) TM0593.pdf (PDF)
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