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THETM GST Antibody [Biotin], mAb, Mouse



Cat. No.
Name
Size   
Price
Selected
A00867-200
THETM GST Antibody [Biotin], mAb, Mouse
200 μg(5x40μg)
$333
A00867-40
THETM GST Antibody [Biotin], mAb, Mouse
40 μg
$74
THETM GST Antibody [Biotin], mAb, Mousecustom antibody production
 THE<sup>TM</sup> GST Antibody [Biotin], mAb, Mouse
Full Name THETM GST Antibody [Biotin], mAb, Mouse
Abbreviated name-1
Mouse Anti-GST-tag [Biotin] mAb;
Documents
 THE<sup>TM</sup> GST Antibody [Biotin], mAb, MouseDocument-MSDS: 4225_20080228042254.PDF (PDF)
 THE<sup>TM</sup> GST Antibody [Biotin], mAb, MouseDocument-MSDS: 3739_20080118034804.PDF (PDF)
Figures
 THE<sup>TM</sup> GST Antibody [Biotin], mAb, Mouse zoom THE<sup>TM</sup> GST Antibody [Biotin], mAb, Mouse
Reference
Description
GenScript THETM GST Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of GST in fusion proteins.
Ig Subclass
IgG1, k
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
THETM GST Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Concentration
40 μg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide and 100 μg, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Recombinant GST protein (26 kD)
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized antibody (40 µg, 100 µg, or 200 µg) with 80 µl, 200 µl, or 400 µl of deionized water to the final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
Fusion Partner
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells
Conjugation
Biotin
Host Species
Mouse
Technical Manual
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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