β-secretase, also called BACE (β-site of APP cleaving enzyme), is an aspartic-acid protease important in the pathogenesis of Alzheimers disease and in the formation of myelin sheaths in peripheral nerve cells. BACE1, also known as memapsin-2, is the major β-secretase for generation of the 40 or 42 amino-acid-long β-amyloid peptides that aggregate in the brain of Alzheimers patients. Extracellular cleavage of APP by BACE1 releases a soluble extracellular fragment and is followed by APP cleavage within its transmembrane domain by γ-secretase. The second cleavage releases the intracellular domain of APP and β-amyloid. The immature form of BACE1 contains an N-terminal signal sequence (residues 1–21) followed by a large catalytic domain, a single transmembrane domain (residues 461–477), and a short cytoplasmic domain (residues 478–501). The signal sequence (1-21) is cleaved from the immature form, yielding the proBACE1 protein, which starts at residue 22. The proBACE1 protein is modified by cleavage of 24 N-terminal residues (aa 22-45), producing the mature BACE1 protein.
GenScript Rabbit Anti-BACE1 (26-45) Polyclonal Antibody is developed in rabbit using a synthetic peptide, IRLPLRSGLGGAPLGLRLPR, corresponding to amino acids 26-45 at the N-terminal of human BACE1. GenScript Rabbit Anti-BACE1 (26-45) pAb is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as 40 μg aliquots at concentration of 0.5 mg/ml.
A synthetic peptide (KLH-conjugated), IRLPLRSGLGGAPLGLRLPR, corresponding to amino acids 26-45 at N-terminal of human BACE1
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1 µg/ml Other applications: user-optimized