Presenilin 1 Antibody, pAb, Rabbit
Cat. No.
Name
Size   
Price
Selected
A00881-40
40 μg
$79
Presenilin 1 Antibody, pAb, Rabbit
Full Name Presenilin 1 Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit anti-PS1 pAb;
Documents
Presenilin 1 Antibody, pAb, RabbitDocument-MSDS: 8317_20081118035648.PDF (PDF)
Presenilin 1 Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
Presenilin 1 Antibody, pAb, Rabbit zoomPresenilin 1 Antibody, pAb, Rabbit
Description
Presenilin 1 (PS1) and presenilin 2 (PS2) are catalytic subunits of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). The other members of the gamma-secretase complex are required to have protease activity. PS1 and PS2 may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It regulates epithelial-cadherin function. Three causative genes have been identified that when mutated lead to presenile Alzheimer's disease: APP (amyloid precursor protein gene), PSEN1, and PSEN2. The presenilin 1 gene has recently been discovered on chromosome 14 and is responsible for an early onset form of Alzheimer's disease that accounts for 70-80% of the inherited forms of Alzheimer's. It is a membrane protein that has a region where at least seven mutations have been identified in family members with Alzheimer's disease. Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (28 kD) and carboxy-terminal (≈17 kD) fragments.
GenScript Rabbit Anti-Presenilin 1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide, TVRSQNDNRERQEHNDRRSLGHPEPL, corresponding to amino acids 25-50 of the N-terminus of human presenilin 1.
Rabbit Anti-Presenilin 1 Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-Presenilin 1 Polyclonal Antibody can detect endogenous levels of the full-length protein (approx. 43-52 kD) and the N-terminal fragment of presenilin 1 (≈ 28 kD).
Formulation
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide
Immunogen
Synthetic peptide corresponding to amino acids 25-50 (TVRSQNDNRERQEHNDRRSLGHPEPL) of the N-terminus of human presenilin 1, which is exposed to the extracellular region, KLH conjugated.
Purification Method
Immunoaffinity chromatography
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2.0 µg/ml
Conjugation
Unconjugated
Species Reactivity
Human, mouse
Host Species
Rabbit
Antigen Species
Human
Note
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
Citation
Okumura N.,et al. Genistein downregulates presenilin 1 and ubiquilin 1 expression.Mol Med Rep.2012Feb;5(2):559-61

Yoshida H.,et al. Turmeric and curcumin suppress presenilin 1 protein expression in Jurkat cells.Exp Ther Med.2011Apr;2(4):629-632

1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.