||Rabbit anti-APH1 pAb;
||Anterior Pharynx-Defective 1 (APH1), in addition to presenilin, PEN2, and Nicastrin forms the γ-secretase protein complex. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. The γ-secretase complex can cleave the beta-amyloid (A4) precursor protein and yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Three mammalian homologues of APH1 have been identified: APH1a, APH1b, and APH1c, of which APH1a is more abundantly expressed than APH1b in human tissue samples. In addition, two isoforms of APH1a can be generated from alternative splicing of APH-1a, APH1a long form (APH1aL) and APH1a short form (APH1aS) that diverge at the C-terminus. APH1a is the major mammalian APH1 isoform required for Notch signaling during embryogenesis and the major APH1 isoform required for the assembly of the γ-secretase complexes and APP processing.
GenScript Rabbit Anti-APH1 Polyclonal Antibody is developed in rabbit using a synthetic peptide, RRQEDSRVMVYSALRIPPED, corresponding to amino acids 246-265 of the C-terminus of human APH1a.
GenScript Rabbit Anti-APH1 Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml.
||Synthetic peptide corresponding to amino acids 246-265 (RRQEDSRVMVYSALRIPPED) of the C-terminus of human APH-1a conjugated to KLH
||0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide
||GenScript Rabbit Anti-APH1 Polyclonal Antibody can detect endogenous levels of human and mouse APH1a.
||The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
||Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.
|Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/ml
Western blot: 0.5-2.0 μg/ml
Other applications: user-optimized
Western Blot Protocol(pdf)
G Ma. et al. Journal of Neuroscience (2005), 25(1):192-198
Subramaniam D.,et al. Curcumin Induces Cell Death in Esophageal Cancer Cells through Modulating Notch Signaling.PLoS One.2012Feb;78(2):e30590