Catalog Products » Antibodies » Primary Antibodies » Control Antibodies » ?-Actin-1 Antibody, pAb, Rabbit
GenScript Share
Cat. No.
Name
Size   
Price
Selected
A00885-40
40 μg
$79 $47
A00885-200
200 μg
$275
α-Actin-1 Antibody, pAb, Rabbitcustom antibody production
α-Actin-1 Antibody, pAb, Rabbit
Full Name α-Actin-1 Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-α-Actin-1 pAb;
Documents
α-Actin-1 Antibody, pAb, RabbitDocument-MSDS: 8335_20081118205951.PDF (PDF)
α-Actin-1 Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
α-Actin-1 Antibody, pAb, RabbitDocument-COA: 13C000595_A00885_COA.pdf (PDF)
α-Actin-1 Antibody, pAb, RabbitDocument-COA: 14A000734_A00885_COA.pdf (PDF)
Figures
α-Actin-1 Antibody, pAb, Rabbit zoomα-Actin-1 Antibody, pAb, Rabbit
α-Actin-1 Antibody, pAb, Rabbit zoomα-Actin-1 Antibody, pAb, Rabbit
Citations
Description
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.
GenScript Rabbit Anti-Alpha-Actin-1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide conjugated to KLH.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-Alpha-Actin-1 Polyclonal Antibody reacts with human, mouse, rat, cow, pig, rabbit, and some fish species. It has not yet been tested with other species.
Concentration
0.5 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Synthetic peptide GQVITIGNERFR conjugated to KLH
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml, if the antibody is in lyophilized form.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml
Western Blot Using the ONE-HOUR WesternTM Kit: For quick results, the GenScript ONE-HOUR WesternTM kit (L00204C) is recommended.
Mix 10 µg of GenScript Rabbit Anti-α-Actin-1 with 10 ml of WB solution for an 8 cm x 8 cm membrane.
Other applications: user-optimized
Conjugation
Unconjugated
Host Species
Rabbit
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Related Products
Cat. No.
Name
Size   
Price
Selected
A00702-100
100 μg
$175
A00702-40
40 μg
$69
A00702-200
200 μg
$311
A00730-40
40 μg
$74
A00730-200
200 μg
$333
A00885-40
40 μg
$79
A00885-200
200 μg
$275
A01546
100 μg
$335
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.