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α-Actin-1 Antibody, pAb, Rabbit


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Cat. No.
Product Name
Size
Price
Quantity
A00885-40 α-Actin-1 Antibody, pAb, Rabbit
40 μg
$99.00
A00885-200 α-Actin-1 Antibody, pAb, Rabbit
200 μg
$349.00



Abbreviated name-1 Rabbit Anti-α-Actin-1 pAb;
Description The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.
GenScript Rabbit Anti-Alpha-Actin-1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide conjugated to KLH.
Immunogen Synthetic peptide GQVITIGNERFR conjugated to KLH
Host Species Rabbit
Conjugation Unconjugated
Purification Method Immunoaffinity chromatography

Formulation 0.5 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml, if the antibody is in lyophilized form.
Ig Subclass Rabbit IgG
Specificity GenScript Rabbit Anti-Alpha-Actin-1 Polyclonal Antibody reacts with human, mouse, rat, cow, pig, rabbit, and some fish species. It has not yet been tested with other species.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml
Western Blot Using the ONE-HOUR WesternTM Kit: For quick results, the GenScript ONE-HOUR WesternTM kit (L00204C) is recommended.
Mix 10 µg of GenScript Rabbit Anti-α-Actin-1 with 10 ml of WB solution for an 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

α-Actin-1 Antibody, PAb, Rabbit
α-Actin-1 Antibody, PAb, Rabbit

Western blot analysis of tissue and cell lysates using α-Actin-1 Antibody, pAb, Rabbit (GenScript, A00885, 1 µg/ml)
The signal was developed with with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 42 kD
Observed Size: 42 kD

α-Actin-1 Antibody, PAb, Rabbit
α-Actin-1 Antibody, PAb, Rabbit

Western blot analysis of tissue and cell lysates using α-Actin-1 Antibody, pAb, Rabbit (GenScript, A00885, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 42 kD
Observed Size: 42 kD

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit

α-Actin-1 Antibody, PAb, Rabbit


Cat. No.
Product Name
Size
Price
Quantity
A00702-100 THETM beta Actin Antibody, mAb, Mouse
100 μg
$175.00
A00702-40 THETM beta Actin Antibody, mAb, Mouse
40 μg
$69.00
A00702-200 THETM beta Actin Antibody, mAb, Mouse
200 μg
$311.00
A00730-40 THETM beta Actin Antibody [HRP], mAb, Mouse
40 μg
$74.00
A00730-200 THETM beta Actin Antibody [HRP], mAb, Mouse
200 μg
$333.00
A00885-40 α-Actin-1 Antibody, pAb, Rabbit
40 μg
$99.00
A00885-200 α-Actin-1 Antibody, pAb, Rabbit
200 μg
$349.00
A01546 THETM beta Actin Antibody [Biotin], mAb, Mouse
100 μg
$180.00


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