Indoleamine 2,3-dioxygenase (IDO) catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated in protection against intracellular and extracellular pathogens.
GenScript Rabbit Anti-IDO Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide conjugated to KLH. GenScript Rabbit Anti-IDO Polyclonal Antibody is highly purified from rabbit antiserum by Protein A.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 0.1-1.0 µg/ml Western Blot Using the ONE-HOUR WesternTM Kit: For quick results, we recommend the GenScript ONE-HOUR WesternTM Kit (L00204C). Mix 10 µg of this antibody with 10 ml of WB solution for an 8 cm x 8 cm membrane. Other applications: user-optimized