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Cre Recombinase Antibody, mAb, Mouse

Cat. No.	Name	Size	Price
A00888-200	Cre Recombinase Antibody, mAb, Mouse	200 μg(5x40μg)	$225
A00888-40	Cre Recombinase Antibody, mAb, Mouse	40 μg	$50

Full Name

Cre Recombinase Antibody, mAb, Mouse

Abbreviated name-1

anti-Cre recombinase mAb;

Documents
Document-MSDS: 8345_20081118211912.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family.
GenScript Mouse Anti-Cre recombinase Monoclonal Antibody is an important tool for the identification of Cre recombinase in different protein expression systems.

Ig Subclass

Mouse IgG2a

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

Negative Control: E. coli. and cell lysate (not mammalian)

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide

Immunogen

Purified recombinant Cre recombinase protein

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Fusion Partner

Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells

Conjugation

Unconjugated

Host Species

Mouse

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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