Cre Recombinase Antibody, mAb, Mouse
Cat. No.
Name
Size   
Price
Selected
A00888-40
40 μg
$79 $47
Cre Recombinase Antibody, mAb, Mousecustom antibody production
Cre Recombinase Antibody, mAb, Mouse
Full Name Cre Recombinase Antibody, mAb, Mouse
Abbreviated name-1
anti-Cre recombinase mAb;
Documents
Cre Recombinase Antibody, mAb, MouseDocument-MSDS: 8345_20081118211912.PDF (PDF)
Cre Recombinase Antibody, mAb, MouseDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
Cre Recombinase Antibody, mAb, Mouse zoomCre Recombinase Antibody, mAb, Mouse
Description
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family.
GenScript Mouse Anti-Cre recombinase Monoclonal Antibody is an important tool for the identification of recombinant Cre recombinase from E.coli expression systems.
Ig Subclass
Mouse IgG2a
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
Recombinant Cre recombinase from E.coli expression systems.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Immunogen
Purified recombinant Cre recombinase protein
Clone ID
1D8H3
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Fusion Partner
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells
Conjugation
Unconjugated
Host Species
Mouse
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
Citation
Lin H.,et al. Establishment and characterization of a tamoxifen-mediated reversible immortalized mouse dental papilla cell line.In Vitro Cell Dev Biol Anim.2013Feb;49(2):114-121

Kaushik S.,et al. Loss of autophagy in hypothalamic POMC neurons impairs lipolysis.EMBO Rep.2012Mar;13(3):258-65

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