PKCδ Antibody, pAb, Rabbit
|A00905-40||PKCδ Antibody, pAb, Rabbit||
|Product Aliases||Rabbit Anti-PKCδ pAb;|
|Description||The protein kinase C (´ PKC ´, EC 184.108.40.206) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups, differing in the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising α, βI (also known as β1), βII (also known as β2) and γ), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation; novel (n)PKC isoforms (comprising δ, ε, η (also known as PKC-L), θ and µ (the mouse homolog of human PKCµ, also known as PKD)) that require DAG but not Ca2+; and atypical (a)PKC isoforms, namely ζ, ι and λ (the mouse homolog of human PKCι) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCν.
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
PKCδ (also known as PRKCD, PKC delta) is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types.
GenScript Rabbit Anti-PKCδ Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human PKCδ.
|Immunogen||Synthetic peptide (KLH-coupled) derived from human PKCδ|
|Purification Method||Immunoaffinity chromatography|
|Formulation||0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide|
|Ig Subclass||Rabbit IgG|
|Specificity||GenScript Rabbit Anti-PKCδ Polyclonal Antibody detects endogenous levels of total PKCδ protein. No cross-reactivity to other PKC isoforms has been detected.|
|Storage||The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Note||Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
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