The protein kinase C (´ PKC ´, EC 22.214.171.124) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups, differing in the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising α, βI (also known as β1), βII (also known as β2) and γ), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation; novel (n)PKC isoforms (comprising δ, ε, η (also known as PKC-L), θ and µ (the mouse homolog of human PKCµ, also known as PKD)) that require DAG but not Ca2+; and atypical (a)PKC isoforms, namely ζ, ι and λ (the mouse homolog of human PKCι) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCν. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKCδ (also known as PRKCD, PKC delta) is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. GenScript Rabbit Anti-PKCδ Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human PKCδ.
Synthetic peptide (KLH-coupled) derived from human PKCδ
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1 µg/ml Other applications: user-optimized
Western blot analysis of E.coli expressed PKC isoforms using Rabbit Anti-PKCδ Polyclonal Antibody (GenScript, A00905), demonstrating the isoform-specificity of this antibody.
Western blot analysis of K562 cell lysates (Lane 1 and 2) and Hela cell lysates (Lane 3 and 4) using Rabbit Anti-PKCδ Polyclonal Antibody (GenScript, A00905), pre-incubated or not pre-incubated with blocking peptide as indicated.