PKCα/β2 Antibody (Phospho-Thr638/641), pAb, Rabbit
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PKCα/β2 Antibody (Phospho-Thr 638/641), pAb, Rabbit  |
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| Full Name |
PKCα/β2 Antibody (Phospho-Thr638/641), pAb, Rabbit
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Abbreviated name-1 |
Anti-PKCα/β2 pT641; |
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Description |
Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups differing in the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising α, βI (also known as β1), βII (also known as β2) and γ)), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation; novel (n)PKC isoforms (comprising δ, ε, η (also known as PKC-L), θ and µ (the mouse homolog of human PKCµ, known as PKD)) that require DAG but not Ca2+; and atypical (a)PKC isoforms, namely ζ, ι and λ (the mouse homolog of human PKC ι) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCν.
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
PKC activity is regulated through three distinct phosphorylation sites, the activation loop threonine (Thr497 in PKCα and Thr500 in PKCβ2), the turn loop autophosphorylation threonine (Thr638 in PKCα and Thr641 in PKCβ2) and the hydrophobic loop serine (Ser657 in PKCα and Ser660 in PKCβ2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-PKCα/β2 (Phospho-Thr638/641) Polyclonal Antibody detects endogenous levels of PKCα only when phosphorylated at threonine 638 and PKCβ2 only when phosphorylated at threonine 641. The reactivity is tested in human and mouse cell/tissue lysates. It has not yet been tested with other species. |
Concentration |
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide |
Immunogen |
Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr638 of human PKCα and Thr641 of human PKCβ2. |
Purification Method |
The antibody is purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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 |
1 |
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Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
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Document-MSDS: 5838_20080917223934.PDF (PDF)
