Catalog Products » Catalog Peptide » V-ATPase Subunit A Antibody, pAb, Rabbit
Manual
Manual
COAs

V-ATPase Subunit A Antibody, pAb, Rabbit

*This product has been discontinued!*
GenScript Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A.Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.
A00938
Ask us a question
Overview
Specificity GenScript Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A.Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.
Host Species Rabbit
Immunogen Synthetic peptide conjugated to KLH
Conjugate Unconjugated
Back

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.02-0.2 µg/ml
Western Blot 1.0 µg/ml
Immunoprecipitation 2-10 µg/mg of lysate
Other applications User-optimized
Back

Properties
Form Liquid
Storage Buffer 1×PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide
Concentration 0.5 mg/ml
Storage Instructions The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap.
Back

Background
Target Background The V-ATPase subunit A is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase.V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.GenScript Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide, purified from rabbit antiserum by immunoaffinity chromatography.
Synonyms Rabbit Anti-V-ATPase Subunit A pAb;
Back

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Zhaotong Wang, et al. Py-FITC: a new fluorescent probe for live cell imaging of collagen-rich tissues and ionocytes. Open Biol. (2021-02)
Gokey JJ, et al. The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish. Developmental biology. (2015-11)
Hennigar SR, et al. Obesity-Induced Inflammation Is Associated with Alterations in Subcellular Zinc Pools and Premature Mammary Gland Involution in Lactating Mice. J Nutr. (2015)
Monteiro J, et al. V-ATPase Proton Pumping Activity Is Required for Adult Zebrafish Appendage Regeneration. PLoS One. (2014-03)
Jesper Nylandsted, et al. ErbB2-associated changes in the lysosomal proteome. Proteomics. (2011-07)
Ahmad A, et al. Role of Vacuolar ATPase in the Trafficking of Renal Type IIa Sodium-phosphate Cotransporter. Cell Physiol Biochem. (2011-06)