|Product Aliases||Rabbit Anti-Estrogen Receptor Beta pAb;|
|Description||The estrogen receptor (ESR) beta is a ligand-activated transcription factor. It is composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen receptor beta is highly homologous to estrogen receptor alpha, and it was initially cloned and characterized from testis.
GenScript Rabbit Anti-Estrogen Receptor Beta Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide, and purified from rabbit antiserum by immunoaffinity chromatography.
|Immunogen||Synthetic peptide conjugated to KLH|
|Purification Method||Immunoaffinity chromatography|
|Formulation||0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide|
|Ig Subclass||Rabbit IgG|
|Specificity||GenScript Rabbit Anti-Estrogen Receptor Beta Polyclonal Antibody reacts with mouse estrogen receptor beta.|
|Storage||The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Note||Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.0125-0.1 μg/ml
Western blot: 1.0 μg/ml
Other applications: user-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
Western blot analysis:
Lane 1: EasyWestern Protein Standard (GenScript, MM0908)
Lane 2: Mouse brain tissue lysate
1 µg/ml Rabbit Anti-Estrogen Receptor Beta Polyclonal Antibody (GenScript, A00939)
Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:6,000)
|Cat. No. A00939|