||Rabbit Anti-Presenilin 2 pAb;
||Presenilin 1 (PS1) and Presenilin 2 (PS2) are catalytic subunits of the gamma-secretase complex, which is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). The other members of the gamma-secretase complex are required to have a protease activity. PS1 and PS2 may play a role in intracellular signaling, gene expression and in linking of chromatin to nuclear membrane. It regulates epithelial-cadherin function. Three causative genes have been identified. Mutation of these genes lead to presenile Alzheimers disease. They are APP (amyloid precursor protein gene), PSEN1 and PSEN2. The PSEN2 gene provides instructions for making a protein named presenilin 2. Presenilin 2 processes certain proteins that are important for transmitting biochemical signals from the cell membrane into the nucleus. In the nucleus, these signals activate particular genes that are important for cell growth and maturation.
GenScript Rabbit Anti-Presenilin 2 Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human Presenilin 2.
||Synthetic peptide (KLH-coupled) derived from human Presenilin 2
||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
||Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
||GenScript Rabbit Anti-Presenilin 2 Polyclonal Antibody detects endogenous levels of total human and mouse Presenilin 2 protein.
||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
|The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized
Western Blot Protocol(pdf)
Okumura N.,et al. Genistein downregulates presenilin 1 and ubiquilin 1 expression.Mol Med Rep.2012Feb;5(2):559-61
Yoshida H.,et al. Turmeric and curcumin suppress presenilin 1 protein expression in Jurkat cells.Exp Ther Med.2011Apr;2(4):629-632