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PKA RIIβ Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A00949-200	PKA RIIβ Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A00949-40	PKA RIIβ Antibody, pAb, Rabbit	40 μg	$50

Full Name

PKA RIIβ Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-PKA RIIβ pAb;

Documents
Document-MSDS: 5834_20080917223331.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
Reference

Description

The cAMP-dependent protein kinase (PKA) is a critical kinase involved in numerous biological functions. The PKA holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. PKA regulatory (PKA R) subunits exist as two classes, RI and RII, with each class containing two isoforms, α and β. Three catalytic subunit isoforms, Cα, Cβ, and Cγ, have been identified. The PKA R subunits allow a range of modifying agents to influence PKA activity and localization, and are found in different levels in a tissue-specific manner. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.
GenScript Rabbit Anti-PKA RIIβ Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human PKA regulatory IIβ subunit (PKA RIIβ).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-PKA RIIβ Polyclonal Antibody detects endogenous levels of human and mouse PKA regulatory subunit RIIβ (PKA RIIβ) protein. It does not cross-react with PKA regulatory subunit peptides derived from PKA RIα, PKA RIβ, or PKA RIIα.

Concentration

0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide

Immunogen

Synthetic peptide (KLH-coupled) derived from human PKA regulatory IIβ subunit (PKA RIIβ)

Purification Method

Immunoaffinity chromatography

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human, mouse

Host Species

Rabbit

Antigen Species

Human

Note

Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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