Catalog Products » Antibodies » antibody : Rabbit Anti-PGK1 Polyclonal Antibody
Cat. No.
Name
Size   
Price
Selected
A00961-40
40 μg
$79 $47
PGK1 Antibody, pAb, Rabbitcustom antibody production
PGK1 Antibody, pAb, Rabbit
Full Name PGK1 Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-PGK1 pAb;
Documents
PGK1 Antibody, pAb, RabbitDocument-MSDS: 8467_20081120022416.PDF (PDF)
PGK1 Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
PGK1 Antibody, pAb, Rabbit zoomPGK1 Antibody, pAb, Rabbit
Description
The PGK1 gene encodes phosphoglycerate kinase-1, which catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.
GenScript Rabbit Anti-PGK1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-PGK1 reacts with human PGK1. Sequence homology predicts that it will also react with mouse, chicken, fruit fly, and zebrafish PGK1.
Concentration
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Immunogen
Synthetic peptide conjugated to KLH
Purification Method
Immunoaffinity chromatography
Predicted Band Size
44 kD
Observed Band Size
44 kD
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.03-0.125 µg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized
Conjugation
Unconjugated
Host Species
Rabbit
Antigen Species
Human
Note
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.