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Amyloid Precursor Protein Antibody (Ab-730), pAb, Rabbit  |
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| Full Name |
Amyloid Precursor Protein Antibody (Ab-730), pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-Amyloid Precursor Protein (Ab-730) pAb; |
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Description |
Amyloid precursor protein (APP) is a type I integral membrane protein with a large N-terminal extracellular domain, a single transmembrane domain, and a short cytoplasmic tail. APP can be sequentially cleaved by beta-secretase and gamma-secretase to release beta-amyloid, which is the main component of senile plaque in patient brains inflicted with Alzheimer's disease.
GenScript Rabbit Anti-Amyloid Precursor Protein (Ab-730) Polyclonal Antibody is developed in rabbit using the synthesized peptide derived from human APP around the phosphorylation site of serine 730 (Y-T-SP-I-H).
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Amyloid Precursor Protein (Ab-730) Polyclonal Antibody can detect endogenous level of mouse APP. Predicted to react with human, rat and bovine APP according to sequence homology. |
Concentration |
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH-conjugated) corresponding to peptide around the phosphorylation site of serine 730 derived from human APP |
Purification Method |
Immunoaffinity chromatography |
Predicted Band Size |
110 kD |
Observed Band Size |
70 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human
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Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8475_20081120030238.PDF (PDF)