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RAGE Antibody, pAb, Rabbit  |
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| Full Name |
RAGE Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-RAGE pAb;Rabbit Anti Advanced glycosylation end product-specific receptor pAb;Rabbit Anti Receptor for advanced glycosylation end-products pAb; |
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Description |
The Receptor for Advanced Glycation Endproducts (RAGE) is a member of the immunoglobin superfamily of cell surface markers. This protein mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. RAGE (SwissProt Q15109) can act as receptor for amyloid beta peptide. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-RAGE Polyclonal Antibody can detect endogenous level of human RAGE. It has not yet been tested with other species. |
Concentration |
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide |
Immunogen |
Synthesized peptide (KLH conjugated) within residues 100-150 of human RAGE protein (Swiss Prot: Q15109) |
Purification Method |
Immunoaffinity chromatography |
Predicted Band Size |
46 kD |
Observed Band Size |
46 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.03-1 µg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 5830_20080917222755.PDF (PDF)