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Runx3 Antibody, pAb, Rabbit  |
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| Full Name |
Runx3 Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti Runx3 pAb; |
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Description |
Runx3 is also known as Core-binding factor, CBF-alpha 3, acute myeloid leukemia 2 protein, Oncogene AML-2, polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha. It binds to the core site of murine leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. Runx3 may be involved in the control of cellular proliferation and/or differentiation.
GenScript Rabbit Anti-Runx3 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide of mouse Runx3. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Runx3 Polyclonal Antibody can detect endogenous level of mouse Runx3. Not yet tested for other species. |
Concentration |
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH conjugated) |
Purification Method |
Immunoaffinity chromatography |
Predicted Band Size |
44 kD |
Observed Band Size |
44 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.03-1 µg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Antigen Species |
Mouse |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8487_20081120204935.PDF (PDF)
