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CCR3 Antibody, pAb, Rabbit  |
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| Full Name |
CCR3 Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-CCR3 pAb; |
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Description |
CCR3 is a member of the seven transmembrane G-protein coupled receptor (GPCR) family, and is a high affinity chemokine receptor for the chemokines eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26) and MCP-4 (CCL13). In addition, it has been reported to bind RANTES, MCP-3 and MCP-4. CCR3 has been shown to exist in blood, brain, lung, bone marrow, kidney, liver, lymph node, placenta, skin, spleen, thymus, thyroid, uterus, and vessel. The agonists for CXCR3 (IP-10, mig, and I-TAC) act as antagonists for CCR3 by competing for the binding of eotaxin to CCR3. CCR3 likely plays an important role in the migration of eosinophils in allergic airway inflammation and asthma. Meanwhile, CCR3 can also serve as a receptor for HIV-1 cell entry.
GenScript Rabbit Anti-CCR3 Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide derived from N-terminal extracellular domain of human CCR3. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-CCR3 Polyclonal Antibody detects human CCR3 protein. It has not yet been tested with other species. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide derived from the N-terminal extracellular domain of human CCR3 |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Immunohistochemistry: 5-10 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 6919_20081019231242.PDF (PDF)
