Cat. No.
Name
Size   
Price
Selected
A00979-40
40 μg
$79 $47
CCR5 Antibody, pAb, Rabbitcustom antibody production
CCR5 Antibody, pAb, Rabbit
Full Name CCR5 Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-CCR5 pAb;
Documents
CCR5 Antibody, pAb, RabbitDocument-MSDS: 7047_20081020213246.PDF (PDF)
CCR5 Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
CCR5 Antibody, pAb, Rabbit zoomCCR5 Antibody, pAb, Rabbit
CCR5 Antibody, pAb, Rabbit zoomCCR5 Antibody, pAb, Rabbit
Description
The CC chemokine receptor 5 (CCR5) is a member of the CC chemokine receptor family. It has the characteristic structure of a 7 transmembrane G protein-coupled receptor (GPCR). CCR5 is a receptor for a number of inflammatory CC chemokines including MIP-1-alpha, MIP-1-beta and RANTES. It transduces a signal by increasing the intracellular calcium ion level and may play a role in the control of granulocytic lineage proliferation or differentiation. It also acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. CCR5 is highly expressed in spleen, thymus, and also in the myeloid cell line THP-1, the promyeloblastic cell line KG-1A and on the CD4+ and CD8+ T-cells. Moreover, recent studies have highlighted the role of CCR5 in a variety of human diseases, ranging from infectious and inflammatory diseases to cancer.
GenScript Rabbit Anti-CCR5 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide derived from extracellular domain of human CCR5.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-CCR5 Polyclonal Antibody detects human CCR5 protein. It has not yet been tested for other species.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
KLH-coupled synthetic peptide derived from extracellular domain of human CCR5.
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Immunohistochemistry: 5-10 µg/ml
Other applications: user-optimized
Conjugation
Unconjugated
Host Species
Rabbit
Antigen Species
Human
Note
Small volume of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.