||Mouse Anti-PCNA mAb; Proliferating Cell Nuclear Antigen
||The proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle. PCNA was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.
GenScript Mouse Anti-PCNA Monoclonal Antibody is developed in mouse using recombinant human PCNA protein.
GenScript Mouse Anti-PCNA is highly purified from mouse ascites by protein A chromatography and is supplied as 40 μg aliquot.
||Recombinant PCNA protein
||Protein A affinity column
||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
||Reconstitute the lyophilized Mouse Anti-PCNA Monoclonal Antibody (40 μg) with 80 μl deionized water to the final concentration of 0.5 mg/ml.
||Mouse IgG1, k
||GenScript Mouse Anti-PCNA Monoclonal Antibody can detect endogenous level of human PCNA. It has not yet been tested with other species.
||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1.0-2.0 µg/ml
Other applications: user-optimized
Western Blot Protocol(pdf)
Yin P., et al. Early growth response-2 expression in uterine leiomyoma cells: regulation and function. Fertil Steril. 2011 Aug;96(2):439-44.