Catalog Products » Antibodies » antibody : Mouse Anti-PCNA Monoclonal Antibody
Cat. No.
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A01055-40
40 μg
$79
PCNA Antibody, mAb, Mouse
Full Name PCNA Antibody, mAb, Mouse
Abbreviated name-1
Mouse Anti-PCNA mAb; Proliferating Cell Nuclear Antigen
Documents
PCNA Antibody, mAb, MouseDocument-MSDS: 5800_20080917035233.PDF (PDF)
PCNA Antibody, mAb, MouseDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
PCNA Antibody, mAb, Mouse zoomPCNA Antibody, mAb, Mouse
Description
The proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle. PCNA was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.
GenScript Mouse Anti-PCNA Monoclonal Antibody is developed in mouse using recombinant human PCNA protein.
GenScript Mouse Anti-PCNA is highly purified from mouse ascites by protein A chromatography and is supplied as 40 μg aliquot.
Ig Subclass
Mouse IgG1, k
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Mouse Anti-PCNA Monoclonal Antibody can detect endogenous level of human PCNA. It has not yet been tested with other species.
Formulation
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Immunogen
Recombinant PCNA protein
Clone ID
4C10G3
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized Mouse Anti-PCNA Monoclonal Antibody (40 μg) with 80 μl deionized water to the final concentration of 0.5 mg/ml.
Predicted Band Size
36 kD
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1.0-2.0 µg/ml
Other applications: user-optimized
Fusion Partner
SP2/0-Ag14
Conjugation
Unconjugated
Host Species
Mouse
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
Citation
Yin P., et al. Early growth response-2 expression in uterine leiomyoma cells: regulation and function. Fertil Steril. 2011 Aug;96(2):439-44.

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