MPS1/RPS27 Antibody, mAb, Mouse
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MPS1/RPS27 Antibody, mAb, Mouse  |
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| Full Name |
MPS1/RPS27 Antibody, mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-MPS1/RPS27 mAb; |
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Description |
Target protein MPS1/RPS27, also known as ribosomal protein S27, RPS27, 40S ribosomal protein S27, metallopanstimulin 1, MPS1, and MPS-1, can catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rats indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Human MPS1/RPS27 has been reported to be 84 amino acids with an apparent molecular weight of 9.5kDa.
GenScript Mouse Anti-MPS1/RPS27 Monoclonal Antibody is developed in mouse using a synthetic peptide conjugated to KLH.
GenScript Mouse Anti-MPS1/RPS27 is highly purified from mouse ascites by protein A chromatography and is supplied as 40 μg aliquot. |
Ig Subclass |
Mouse IgG1, k |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Mouse Anti-MPS1/RPS27 Monoclonal Antibody can detect endogenous level of human MPS1/RPS27. It has not yet been tested with other species. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
Immunogen |
Synthetic peptide conjugated to KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
Predicted Band Size |
9.5 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-0.5 µg/ml
Western blot: 1.0-2.0 µg/ml
Other applications: user-optimized |
Fusion Partner |
SP2/0-Ag14 |
Conjugation |
Unconjugated |
Host Species |
Mouse |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 5802_20080917035738.PDF (PDF)