Human CT-1 Antibody (6F7F9), mAb, Mouse
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Human CT-1 Antibody (6F7F9), mAb, Mouse  |
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| Full Name |
Human CT-1 Antibody (6F7F9), mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-CT-1 mAb; Cardiophin 1 antibody |
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Description |
Cardiotrophin-1 (CT-1) is a member of the cytokine family, which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotropic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower level of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor β subunit. A third α receptor subunit has recently been implicated in the receptor complex.
GenScript Human CT-1 Antibody (6F7F9), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified full length human recombinant CT-1 protein (Swiss Prot: Q16619). |
Ig Subclass |
IgG1 κ |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
React with Human CT-1, not yet tested in other species |
Concentration |
1 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
purified full length human recombinant CT-1 protein (Swiss Prot: Q16619) |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
Predicted Band Size |
21.5 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 0.5-10 µg/ml
ELISA Detection: 0.05-0.2 μg/ml
Recommended antibody pairing for sandwich immunoassay: |
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Western Blot: 0.5-1.0 µg/ml
Other Applications: user-optimized |
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Fusion Partner |
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
Conjugation |
Unconjugated |
Host Species |
Mouse |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 5804_20080917041254.PDF (PDF)