PKCν Antibody, pAb, Rabbit
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PKCν Antibody, pAb, Rabbit  |
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| Full Name |
PKCν Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-PKCν pAb; |
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Description |
Protein kinase C (PKC, EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. It is involved in diverse cellular signaling pathways. Specifically, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
PKC family is divided into three groups based on different enzymes cofactor requirements. They are conventional (c) PKC isoforms, novel (n) PKC isoforms, and atypical (a)PKC isoforms respectively. Conventional (c) PKC isoforms consisting α, βI, βII and γ require diacylglycerol (DAG), Ca2+, and phospholipid for activation. Novel (n)PKC isoforms consisting δ, ε, η (also known as PKC-L), θ and µ(the mouse homolog of human PKCµ, known as PKD) require DAG but not Ca2+ for activation. Atypical (a)PKC isoforms, consisting ζ, ι and λ (the mouse homolog of human PKC ι) require neither Ca2+ nor DAG. Other than those listed above, a new PKC member has recently been discovered and is referred to as PKCν. PKCν (PKC nu), also known as Protein kinase D3 (PKD3), is widely expressed in human and murine tissue.
It is stimulated by both phorbol ester and growth factors and is phosphorylated upon stimulation at Ser731 and Ser735, located within the activation loop. PKD3 continuously shuttles between the nucleus and the cytoplasm, which requires a functional CRM1 nuclear export pathway.
GenScript Rabbit Anti-PKCν Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human PKCν. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-PKCν Polyclonal Antibody detects endogenous human and mouse PKCν protein. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH-coupled) derived from human PKCν |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 6572_20081013221834.PDF (PDF)