HDAC2 (Histone deacetylase 2) belongs to the histone deacetylase family. Histone deacetylases are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3, and H4) via the formation of large multiprotein complexes. Histone deacetylation forms the tagging for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. HDAC2 also forms transcriptional repressor complexes by associating with many different proteins, such as YY1, a mammalian zinc-finger transcription factor.
GenScript Rabbit Anti-HDAC2 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide derived from the carboxyl terminal residues of the human HDAC2 protein (Swiss Prot: Q92769).
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
GenScript Rabbit Anti-HDAC2 Polyclonal Antibody detects endogenous levels of human HDAC2 protein.
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.5-1 µg/ml Other applications: user-optimized
Western blot analysis of Hela cell lysate using 1 µg/ml Rabbit Anti-HDAC2 Polyclonal Antibody (GenScript, A01178) Lane 1. Rabbit Anti-HDAC2 Polyclonal Antibody Lane 2. Rabbit Anti-HDAC2 Polyclonal Antibody pre-incubated with immunizing peptide The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.