Vimentin Antibody, pAb, Rabbit

*This product has been discontinued!*

Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments form stable structures, in fibroblasts, vimentin exists as a dynamic structure.GenScript Rabbit Anti-Vimentin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 300-350 of human vimentin protein (Swiss Prot: P08670).

Cat. No.	A01189
Size	Buy in bulk

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Overview Applications Properties Background

Overview

Specificity	GenScript Rabbit Anti-Vimentin Polyclonal Antibody detects endogenous levels of human vimentin protein.
Host Species	Rabbit
Immunogen	Synthetic peptide (KLH-coupled) derived from within residues 300-350 of human vimentin (Swiss Prot: P08670)
Species Reactivity	Human. This product has not yet been tested with other species.

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Applications

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.5-1.0 µg/ml
Immunohistochemistry	5-10 µg/ml
Other applications	User-optimized

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Properties

Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Purification	Immunoaffinity chromatography

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Background

Target Background

Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments form stable structures, in fibroblasts, vimentin exists as a dynamic structure.
GenScript Rabbit Anti-Vimentin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 300-350 of human vimentin protein (Swiss Prot: P08670).

Synonyms

Rabbit anti Vimentin polyclonal antibody;

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Citations

1. Wang J, et al. Enhancer of zeste homolog 2 blockade by RNA interference is implicated with inhibited proliferation, invasion and promoted apoptosis in endometrial carcinoma. Oncol Lett. (2018)
2. Lu M, et al. The high expression of long non-coding RNA PANDAR indicates a poor prognosis for colorectal cancer and promotes metastasis by EMT pathway. J Cancer Res Clin Oncol. (2017)
3. Ihira Kei, et al. EZH2 inhibition suppresses endometrial cancer progression via miR-361/Twist axis. Oncotarget. (2017)
4. Dong P, et al. Identification of KLF17 as a novel epithelial to mesenchymal transition inducer via direct activation of TWIST1 in endometrioid endometrial cancer. Carcinogenesis. (2013-11)
5. Dong P, et al. Mutant P53 Gain-Of-Function Induces Epithelial-Mesenchymal Transition Through Modulation Of The Mir-130B-Zeb1 Axis. Oncogene. (2012-07)