Vimentin Antibody, pAb, Rabbit
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Vimentin Antibody, pAb, Rabbit  |
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| Full Name |
Vimentin Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit anti Vimentin polyclonal antibody; |
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Description |
Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments form stable structures, in fibroblasts, vimentin exists as a dynamic structure.
GenScript Rabbit Anti-Vimentin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 300-350 of human vimentin protein (Swiss Prot: P08670). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Vimentin Polyclonal Antibody detects endogenous levels of human vimentin protein. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH-coupled) derived from within residues 300-350 of human vimentin (Swiss Prot: P08670) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
53 kD |
Observed Band Size |
53 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human. This product has not yet been tested with other species. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8559_20081123202513.PDF (PDF)
