| Full Name |
Mouse Anti-DYKDDDDK-tag Monoclonal Antibody, IgG2b Subclass
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Abbreviated name-1 |
Mouse Anti-DYKDDDDK-tag mAb |
| Documents |
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 MSDS: 20081224220609 (PDF) |
| MSDS: 20080118034804 (PDF) |
| Figures |
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| Reference |
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Hu D. et al. c-Jun N-terminal kinase 1 interacts with and negatively regulates Wnt/beta-catenin signaling through GSK3beta pathway. Carcinogenesis. Dec 2008;29(12):2317-24.
Chenere P. Ramsey, et al. The E163K DJ-1 mutant shows specific antioxidant deficiency. Brain Res. Sep 16. 2008.
Kimura M, et al. Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II. Mol Cell Biol. Mar 2002;22(5):1577-1588.
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Description |
Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a purified IgG2b/kappa monoclonal antibody isolated from murine ascites fluid. It can be used to detect DYKDDDDK-tagged proteins. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility for it to interfere with protein expression, proteolytic maturation, antigenicity and other functions. The enterokinase cleavage site allows it to be completely removed from purified fusion proteins.
GenScript Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is also useful for the affinity purification of proteins with DYKDDDDK markers at free N-termini or C-termini when bound to a solid support.
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Key Features |
High Sensitivity |
Ig Subclass |
Mouse IgG2b |
Storage |
This product should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
The antibody recognizes C-terminally, N-terminally, and internally DYKDDDDK-tagged fusion proteins. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Clone |
A01190.01 |
Immunogen |
A KLH coupled synthetic peptide (DYKDDDDK) |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody (40 μg or 100 μg) with 80 μl or 200 μl of deionized water to the final antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
49 kDa |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunoprecipitation: 1 µg/ml
Other applications: user-optimized |
Fusion Partner |
Spleen cells fused with SP2/0-Ag14 mouse myeloma cells. |
Host Species |
Mouse |
Note |
Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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